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. Author manuscript; available in PMC: 2016 Jun 15.
Published in final edited form as: Bioorg Med Chem. 2015 Mar 17;23(12):2828–2838. doi: 10.1016/j.bmc.2015.03.027

Figure 6.

Figure 6

Purified P87C PTP1B and P87C/A122C PTP1B exhibit marginal differences in FlAsH sensitivity. (A) The phosphatase activities of purified wild-type and engineered PTP1B enzymes (50 nM) were measured with pNPP in the presence of the indicated FlAsH concentrations after 120-minute pre-incubations. The activities were normalized to DMSO-only controls for the corresponding enzymes. (B) The PTP1B mutants’ rates of dephosphorylating pNPP in the presence of 500 nM FlAsH over 1-minute windows were normalized to a DMSO-only control. The results of three independent experiments (grayscale for P87C PTP1B, redscale for P87C/A122C PTP1B) were averaged and the averaged data was fitted as single-variable exponential decays.