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. 2015 Apr 3;308(11):H1323–H1335. doi: 10.1152/ajpheart.00654.2014

Fig. 1.

Fig. 1.

J. C. Russel (JCR) rats were treated with anti-microRNA-21 (miR-21) or scrambled (nontargeting) anti-miR on day 5 repetitive ischemia (RI) where indicated, and Sprague-Dawley (SD) and JCR rats were euthanized on days 0, 3, 6, or 9 of RI (n = 6). Tissue samples were collected from normal zone (NZ) and collateral-dependent zone (CZ). Top: cumulative data (n = 6). Bottom: representative Western blots from CZ. A: anti-Bcl-2. B: anti-phospho (p)-(Ser155)-Bad. C: anti-Bax. D: anti-Bcl-2 or anti-Bax antibodies were used for immunoprecipitation (IP) as indicated. Samples were then subject to SDS-PAGE, followed by Western blot analysis with anti-Bax antibodies. The same blot was stripped and reprobed for Bcl-2, p(Ser115)Bad, total Bad, Bax, and β-tubulin. E: tissue samples were collected from CZ and NZ, and mitochondria were separated from the cytosol. Cytochrome c was detected by Western blot analysis. The cytoplasmic sample was stripped and reprobed with β-tubulin. *P < 0.05 vs. sham; #P < 0.05 vs. Wistar-Kyoto (WKY) RI; $P < 0.05 vs. JCR RI.