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. 2015 Apr 3;308(11):H1323–H1335. doi: 10.1152/ajpheart.00654.2014

Fig. 4.

Fig. 4.

Fig. 4.

SD and JCR rats were treated with anti-miR-21 or scrambled anti-miR on day 5 RI or with anti-CD11b/CD18 and CD44 blocking antibodies on days 6–9 RI as indicated and euthanized on days 6 (12 h post-blocking antibody administration) or 9 RI, or untreated and euthanized on days 0, 3, 6, and 9 of RI. A–D: flow cytometry was performed on CZ and NZ perfusates using Gr-1 (Ly6G) antibodies to label neutrophils, CD115 antibodies to label monocytes, and annexin V antibodies to label apoptotic cells. Representative analysis (A and B) and cumulative data (C and D) are shown. *P < 0.05 vs. sham; #P < 0.05 vs. WKY RI; $P < 0.05 vs. JCR RI. E and F: representative hematoxylin-and-eosin images (×100) of remodeling collateral arteries (left) and large preexisting arteries (right) in cardiac cross sections from unperfused (E) and perfused (F) JCR rat hearts on day 9 RI. White arrows point to neutrophils. Note differences in size bars.