Fig. 9.

Cell-surface marker characterization of primary cultures of human esophageal stromal cells. A: forward-scatter-area (FSC-A) and side-scatter-area (SSC-A) of cells grown in primary culture are shown. A total of 89.2% of events was gated. Stromal cell marker CD90 is present on the majority (97.7%) of human esophageal stromal cells in primary culture (B). Esophageal stromal cells in primary culture cells lack expression of hematopoietic CD45 (C), endothelial CD31 (D), and epithelial CD324 markers (E). Positive controls included commercially purchased human mononuclear cells (HemaCare). Isotype controls were used to determine nonspecific staining for each antibody.