Fig. 2.

Treatment of OVE26 mice with GKT137831 reduces diabetes-induced extracellular matrix protein expression in the renal cortex including glomeruli. A: representative Western blot for fibronectin protein expression in the renal cortex. Representative results of Western blot analysis were obtained from 3 independent samples from each group. Each histogram at the bottom represents the ratio of the intensity of the fibronectin bands quantified by densitometry factored by the densitometric measurement of the actin band. Values are means ± SE from all the animals in each group. *P < 0.05 vs. FVB. #P < 0.05 vs. OVE26. B: representative Western blot for collagen IV protein expression in the renal cortex. Representative results of Western blot analysis were obtained from 2 independent samples from each group. The histogram on the right represents the ratio of the intensity of collagen IV bands quantified by densitometry factored by the densitometric measurement of GAPDH band. The data are expressed as percentage of control (FVB), where the ratio in the control was defined as 100%. Values are means ± SE from all the animals in each group. *P < 0.05 vs. FVB. #P < 0.05 vs. OVE26. C: fibronectin expression was detected by immunoperoxidase (top) and immunofluorescence (bottom) staining of kidney sections from FVB, OVE26, OVE26+GKT137831 10 mg/kg, and OVE26+GKT137831 40 mg/kg groups. D: quantitation of glomerular collagen fibronectin expression. The histograms represent means ± SE of glomeruli in sections from individual mice in each group. *P < 0.001 vs. FVB. #P < 0.001 vs. OVE26. E: collagen type IV was detected by immunoperoxidase (top) and immunofluorescence (bottom) staining of kidney sections from FVB, OVE26, OVE26+GKT137831 10 mg/kg, and OVE26+GKT137831 40 mg/kg groups. F: quantitation of glomerular collagen type IV expression. The histograms represent means ± SE of glomeruli in sections from individual mice in each group. *P < 0.001 vs. FVB. #P < 0.001 vs. OVE26.