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. 2015 Mar 18;308(11):C899–C918. doi: 10.1152/ajpcell.00002.2015

Fig. 9.

Fig. 9.

Effect of TTA-P2 on ACTH- and 8-BrcAMP-induced cortisol secretion from bovine AZF cells. At 24 h after bovine AZF cells were plated, medium was aspirated and replaced with defined medium without (control) or with ACTH, TTA-P2, or 8-BrcAMP. Samples containing TTA-P2 were pretreated with TTA-P2 alone for 30 min prior to addition of ACTH or 8-BrcAMP. Medium was collected at 1, 2, and 24 h, and cortisol concentration was determined by enzyme immunoassay. Values are means ± SE of duplicate determinations from triplicate plates. A: cortisol from media samples collected 1 h and 24 h after no treatment (control) or treatment with ACTH (2 nM), TTA-P2 (1,250 nM), or ACTH (2 nM) + TTA-P2 (10-1,250 nM). P < 0.001 for ≥50 nM TTA-P2 at 1 and 24 h. B: cortisol from media samples collected 1 h after no treatment (control) or treatment with TTA-P2 (500 nM) or ACTH (0.001–10 nM) + TTA-P2 (500 nM). P < 0.0003 for TTA-P2 inhibition at each ACTH concentration. C: cortisol in media samples collected 2 h and 24 h after no treatment (control) or treatment with 8-BrcAMP (600 μM), TTA-P2 (10-1,250 nM), or 8-BrcAMP (600 μM) + TTA-P2 (1,250 nM). At 2 h, P < 0.0001 for >50 nM TTA-P2; at 24 h, P < 0.003 for all TTA-P2 concentrations.