Skip to main content
. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Immunol Methods. 2015 Apr 17;421:112–121. doi: 10.1016/j.jim.2015.04.004

Figure 6. Evaluation of biases in the sequences comprising the four databases.

Figure 6

A) Mapping efficacy among four databases using forward and reverse ITS amplicon reads. The mapping percentages of forward and reverse ITS reads were calculated for each database using a representative mouse sample from each of four different body sites as indicated. The ratio of sequences matching the forward strand versus the sequences matching the reverse strand was calculated and displayed. All libraries show some bias for the forward strand. B) ITS sequence completeness was evaluated in the four databases as described in Materials and Methods.