Fig. 2.

Examples of interneuron identification and marking of microinjection site. A and B: Phrenic motoneurons (PMN; A, red) and interneuron (B) identification using spike-triggering averaging (STA) techniques (see Marchenko and Rogers, 2007), demonstrating the presence or absence of a STA peak in the PN, respectively. Black trace in A: STA after shifting PN record by one respiratory cycle. C: histological identification of the siRNA microinjection site using 2% Pontamine sky blue (10 nl). Scale bar is 250 μm.