Fig. 4.

Dynamic changes in phrenic nerve phasic (inspiratory) and tonic background (expiratory) activity during anti-GAD65/67 siRNA microinjection. Across-animal normalized (in % to control preinjection state) inspiratory (A, top) and expiratory (C, top) integrated PN activity ipsilateral to siRNA injection at 0 (black), 45 (blue), 60 (cyan), 75 (green), 90 (orange), 105 (pink) and 120 (red) min following siRNA injection. A, middle: same as top, but with control values subtracted. A and C, bottom: bin P values with significance level of P < 0.05, compared with control. B, same as A, except that expiratory background activity has been subtracted. For correct averaging, respiratory phases were divided in 15 bins to avoid their differences in length in individual animals and across animal set (see Electrophysiology).