Compressed record of phrenic nerve activity before and during anti-GAD65/67 siRNA monolateral microinjection into the phrenic nucleus. Traces, from top to bottom: integrated contralateral (contra, untreated right side, PNR) and ipsilateral (ipsi, treated left side, PNL) PN discharges. BP, blood pressure, TP, tracheal pressure, CO2, end-tidal CO2 level. Arrow indicates beginning of siRNA microinjection.