TABLE 2.
Quantitative and reverse transcriptase quantitative-PCR standard curve descriptors.
| Phylotype | Template | r2 | y-intercept | E (%) | Ct-cutoff |
|---|---|---|---|---|---|
| 16S rRNA | DNA | 0.998 | 35.1 | 92.6 | 28.9 |
| narG-1 | DNA | 0.998 | 41.1 | 77.6 | 29.9 |
| narG-2 | DNA | 0.998 | 43.4 | 85.4 | ND |
| napA-1 | DNA | 0.994 | 44.7 | 95.5 | 32.3 |
| napA-2 | DNA | 0.999 | 42.2 | 86.9 | 31.3 |
| napA-3 | DNA | 0.997 | 41.9 | 89.9 | 34.1 |
| nirS-ef | DNA | 0.998 | 35.0 | 86.0 | 35.9 |
| nirS-m | DNA | 0.996 | 41.1 | 86.0 | ND |
| nirS-n | DNA | 0.998 | 42.8 | 82.1 | ND |
| nrfA-2 | DNA | 0.997 | 39.9 | 96.0 | 28.5 |
| narG-2 | cDNA | 0.999 | 43.3 | 83.0 | ND |
| nirS-ef | cDNA | 0.998 | 43.0 | 85.0 | ND |
E, amplification efficiency; ND, no template control not detected.