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. 2015 Jun 2;6:547. doi: 10.3389/fmicb.2015.00547

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Copyright © 2015 Padilla, Ganesh, Gantt, Huhman, Parris, Sarode and Stewart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chao1 estimates of operational taxonomic unit (97% similarity cluster) richness in prefilter (>1.6 μm; A,B) and Sterivex (0.2–1.6 μm; C,D) samples in experiments 1 and 2. Boxplots show medians within first and third quartiles, with whiskers indicating the lowest and highest values within 1.5 times the interquartile range of the lower and upper quartiles, respectively. Note variation in y-axis scales. Asterisks in (D) indicate volume groupings with richness estimates significantly different from those of the 5 L sample (P < 0.05). All other pairwise differences between volume groupings were not statistically significant.