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. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: Oncogene. 2014 Dec 1;34(35):4591–4600. doi: 10.1038/onc.2014.384

Figure 6. The Kv1.5-WT channel mediates cell death through caspase-3 activation.

Figure 6

(A) Immunocytochemistry and confocal microscopy of Kv1.5-WT expressing TC-71 cells shows induction of caspase-3 cleavage in Kv1.5+ (GFP+) cells (Indicated by white arrows) following exposure to hypoxia. No significant cleavage is detected in Kv1.5+ cells in ambient conditions. (B) Cleaved caspase-3 staining is not detected in parent TC-71 cells in either ambient or hypoxic conditions. To serve as a positive control for cleaved caspase-3 staining, cells were exposed to 4 µg Etoposide. This exposure resulted in robust induction of caspase-3 cleavage in both ambient and hypoxic conditions. Quantification of cells with cleaved caspase-3 is shown for cells in A & B, under ambient (C) (21% O2) and hypoxic (1%O2) (D) conditions. ** p<0.005 (mean ± SEM, n=2–3. 150–400 cell nuclei were counted for each condition).