Table 2.
Results of Welch’s t test comparing the means of Vandana and 481-B for the 20 metabolites, total amino acids, carbohydrates, C, N, and C/N analyzed in root tissue
| Component | n | MeanV | SDV | Mean481B | sd481B | t | df | p value | CI.95 | Tukey HSD | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| His | 6 | 11.81 | 4.61 | 58.97 | 5.75 | 11.08 | 3.82 | 0.000 | 35.12 | 59.21 | *** |
| Asn | 6 | 59.98 | 2.67 | 184.96 | 84.36 | 2.56 | 2.00 | 0.124 | −84.28 | 334.23 | |
| Ser | 6 | 5.56 | 1.26 | 24.52 | 8.21 | 3.95 | 2.09 | 0.054 | −0.81 | 38.74 | ** |
| Gln | 6 | 4.82 | 2.21 | 20.83 | 2.90 | 7.61 | 3.74 | 0.002 | 10.00 | 22.01 | *** |
| Gly | 6 | 1.20 | 0.45 | 1.62 | 0.57 | 1.01 | 3.79 | 0.374 | −0.76 | 1.60 | |
| Asp | 6 | 6.54 | 0.28 | 20.87 | 3.71 | 6.68 | 2.02 | 0.021 | 5.19 | 23.46 | * |
| Glu | 6 | 4.28 | 1.10 | 8.68 | 0.62 | 6.04 | 3.14 | 0.008 | 2.14 | 6.66 | *** |
| Thr | 6 | 13.27 | 3.26 | 34.47 | 16.08 | 2.24 | 2.16 | 0.145 | −16.75 | 59.14 | ** |
| Ala | 6 | 4.98 | 0.57 | 20.02 | 7.06 | 3.68 | 2.03 | 0.065 | −2.35 | 32.42 | ** |
| GABA | 6 | 2.52 | 0.39 | 6.60 | 2.18 | 3.19 | 2.13 | 0.079 | −1.12 | 9.27 | |
| Pro | 6 | 46.65 | 16.35 | 143.67 | 16.41 | 7.26 | 4.00 | 0.002 | 59.89 | 134.14 | *** |
| Lys | 6 | 14.36 | 2.60 | 52.84 | 13.61 | 4.81 | 2.15 | 0.035 | 6.22 | 70.75 | *** |
| Val | 6 | 5.03 | 1.13 | 22.50 | 3.46 | 8.32 | 2.43 | 0.008 | 9.80 | 25.14 | ** |
| Ile | 6 | 1.82 | 0.17 | 11.44 | 2.41 | 6.91 | 2.02 | 0.020 | 3.68 | 15.57 | ** |
| Leu | 6 | 1.22 | 0.15 | 4.79 | 1.30 | 4.70 | 2.05 | 0.040 | 0.38 | 6.74 | *** |
| Phe | 6 | 2.68 | 0.21 | 15.18 | 5.26 | 4.11 | 2.01 | 0.054 | −0.54 | 25.54 | *** |
| Total aa | 6 | 9.27 | 0.66 | 27.68 | 6.38 | 4.97 | 2.04 | 0.037 | 2.79 | 34.04 | *** |
| Starch | 6 | 0.50 | 0.11 | 0.74 | 0.20 | 1.83 | 3.07 | 0.162 | −0.17 | 0.64 | |
| Glucose | 6 | 4.44 | 0.75 | 9.95 | 1.33 | 6.24 | 3.14 | 0.007 | 2.77 | 8.24 | *** |
| Fructose | 6 | 7.06 | 1.21 | 13.70 | 2.20 | 4.58 | 3.10 | 0.018 | 2.11 | 11.18 | *** |
| Sucrose | 6 | 8.52 | 0.76 | 12.42 | 0.86 | 5.88 | 3.95 | 0.004 | 2.05 | 5.75 | *** |
| N | 6 | 1.03 | 0.23 | 1.43 | 0.29 | 1.90 | 3.81 | 0.134 | −0.20 | 1.00 | |
| C | 6 | 0.84 | 0.02 | 0.92 | 0.05 | 2.64 | 2.73 | 0.086 | −0.02 | 0.17 | *** |
| C/N | 6 | 0.84 | 0.19 | 0.66 | 0.10 | −1.53 | 2.95 | 0.225 | −0.58 | 0.21 | |
Columns represent (from left to right): components; total number of samples analyzed (n); mean and standard deviation (SD) for Vandana and 481B, respectively (columns 3–6); Welch’s t value (t); degrees of freedom (df); p value; and the confidence interval at 95 % probability (CI.95). The values used for the calculation are the ratio of each component’s content in drought versus well-watered conditions, representing the genotypic response under drought. The last column represents the significance level obtained from the Tukey HSD test that compared Vandana, 481B, and Way Rarem (significance values are *, **, and *** representing 0.05, 0.01, and <0.001, respectively). Gray-shaded cells are components significant by Welch’s t test at p < 0.05. The comparison between the Welch’s t test and the Tukey HSD highlighted differences in the significance of some components