Table 1.
Primer information for molecular assays used to diagnose clinical samples, produce complete S segment sequence data and monitor isolation attempts.
| Primer pairs | Sequence | Positiona (strand) | Product size | Comment |
|---|---|---|---|---|
| HAN-L-F1 HAN-L-R1 |
ATGTAYGTBAGTGCWGATGC AACCADTCWGTYCCRTCATC |
L 2940 (sense) L 3391 (antisense) |
451 base pairs | Pan-hanta assayb 1st round (nested) |
| HAN-L-F2 HAN-L-R2 |
TGCWGATGCHACIAARTGGTC GCRTCRTCWGARTGRTGDGCAA |
L 2951 (sense) L 3340 (antisense) |
389 base pairs | Pan-hanta assayb 2nd round (nested) |
| CHOV S 1 F CHOV S 719 R |
TAGTAGTAGACTCCTTGAGAAGC CCAAAACCGATGACACCC |
S 1 (sense) S 719 (antisense) |
719 base pairs | CHOV sequencing Primers set 1 |
| CHOV S 472 F CHOV S 1157R |
AGAGGGAGGCAGACTGTGA CCCATAGACTGTGTCCTTCG |
S 472 (sense) S 1157 (antisense) |
685 base pairs | CHOV sequencing Primers set 2 |
| CHOV S 901 F CHOV S 1480 R |
GCTGAGTCTGAAGGTGCC CCCTTAACCCTAAATTAGTGC |
S 901 (sense) S 1480 (antisense) |
579 base pairs | CHOV sequencing Primers set 3 |
| CHOV S 1260F CHOV S 1972R |
GCAGTTAGCACAGTCCTTAGTTG TAGTAGTATGCTCCTTGAAAAGC |
S 1260 (sense) S 1972 (antisense) |
712 base pairs | CHOV sequencing Primers set 4 |
| CHOV S901 F CHOV S1000R CHOV S933P |
GCTGAGTCTGAAGGTGCCAC ATAGTGCTGTTGGTGGACA FAM- CATTGCTGTCCCTCAC TCTGTGTGG -BHQ1 |
S 901 (sense) S1000 (antisense) S933 (sense) |
100 base pairs | CHOV real-time |