Table 1.
Relative amounts (in %) of main N-glycan structures detected in total soluble proteins (TSP) and intercellular fluid (IF) of the different N. benthamiana lines. Analysis was performed in wild type (WT) plants and ΔXT/FT glycosylation mutant (ΔXF), which served as a control. The following STGalT expressing plants were analyzed: P2 of STGalT-WT and of STGalTX−-ΔXF and NP of STGalT-ΔXF. Other: refers to mainly oligomannosidic glycans. As N-glycan profiles of P1 plants did not differ from P2 they are not listed separately in this table. See also Fig. 2 and Supplementary Fig. S2.
| WT |
STGalT-WT (P2) |
ΔXF |
STGalTX−-ΔXF (P2) |
STGalT-ΔXF (NP) |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TSP | IF | TSP | IF | TSP | IF | TSP | IF | TSP | IF | |
| MM | 5.9 | 22 | 19 | 46.2 | 25 | 46.7 | 26.2 | 46.2 | ||
| MMX | 6.3 | 13.2 | 13.8 | 37.9 | ||||||
| MMXF | 17.8 | 27.5 | 7.9 | |||||||
| MGn | 5.3 | 16.7 | 24.3 | 29.5 | 29 | 26.5 | 26.2 | 29.8 | ||
| MGnX | 7.5 | |||||||||
| MGnXF | 19.2 | 16.8 | 5.5 | |||||||
| GnGn | 31.7 | 24.3 | ||||||||
| GnGnF | 13 | |||||||||
| GnGnXF | 45.6 | 42.5 | ||||||||
| MA | 5.7 | 17.5 | 9.8 | 17 | 9.5 | |||||
| MAX | 11 | |||||||||
| MAXF | 9 | |||||||||
| Man4A | 7 | 12.9 | 8.2 | 7.4 | 7.8 | 8 | ||||
| Man5A | 6 | 5.6 | 6.6 | |||||||
| ∑other | 11.1 | – | 15.4 | 10.5 | 12 | – | 14.7 | 9.6 | 16.2 | – |