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. 2015 Jun 2;5:10691. doi: 10.1038/srep10691

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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N. crassa was cultured in Vogel’s medium containing 1% (w/v) glucose and 0.08% (v/v) polyacrylic acid (MW ~100,000) at 25 °C. Reactors were exposed to light for 24hrs following inoculation then maintained in constant darkness for the remainder of the experiment for data collection. The shaded region corresponds to the dark period. Gene expression of (a) frequency (frq), (b) white collar-1 (wc-1), (c) white collar-2 (wc-2), (d) vivid (vvd), and clock-controlled genes (e) ccg-1, (f) ccg-4, and (g) ccg-14 were measured over time and were analyzed using the ΔΔC_T method with btl and vma2 as reference genes. Shown are the data collected from one of three biological replicates, and error bars represent SD between triplicate samples within one biological replicate.