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. 2015 Jun 2;6:520. doi: 10.3389/fmicb.2015.00520

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Copyright © 2015 Catta-Preta, Brum, da Silva, Zuma, Elias, de Souza, Schenkman and Motta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell cycle in control cells. The top panels show optical microscopy images of A. deanei (A) and S. culicis (B) observed by differential interference contrast (DIC) and fluorescence microscopy after staining with DAPI and anti-porin, a specific antibody that labels the symbiont. The black arrow indicates the flagellum. The bottom panels show the flow cytometry histograms of wild-type, aposymbiotic strains and the merged analyses of A. deanei (C–E) and S. culicis (F–H). The sizes of the scale bars are indicated in each figure.