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. 2015 Jun 2;6:520. doi: 10.3389/fmicb.2015.00520

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Copyright © 2015 Catta-Preta, Brum, da Silva, Zuma, Elias, de Souza, Schenkman and Motta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The top panel shows flow cytometry histograms of control A. deanei (A) and S. culicis (D), or cells treated for 24 h with 10 μM camptothecin (B,E). Merged histograms are represented on the right (C,F). The bottom panel represents the cell pattern distribution generated by counting DNA-containing structures of A. deanei (G) and S. culicis (H) after the indicated treatments. F, flagellum; K, kinetoplast; N, nucleus; S, symbiont. S∞—a single symbiont in rod shape per cell, S∞—a single symbiont in constriction (dividing format) per cell, S∞∞—two symbionts in rod shape per cell, S∞∞∞—filamentous symbiont.