Figure 2. Astrocytes increase cystine uptake following IL-1β treatment.

Astrocytes (A; n=4), neurons (B; n=4–8) and microglia (C; n=6–10) were treated with vehicle (white bars) or IL-1β (3 ng/ml; black bars) for 20–24 hr following which cells were washed and incubated with a buffer containing ¹⁴C-L-cystine (3 μM) and uptake was determined over time as indicated. Data are expressed as mean ± SEM ¹⁴C-L-cystine uptake in pmol/mg protein. An asterisk (*) indicates a significant between-group difference as determined by a two-way ANOVA followed by Bonferroni’s post hoc test. Significance was set at p < 0.05.