Figure 6. Protein synthesis is required for the enhancement of astrocyte system x_c⁻ activity that follows IL-1β treatment.

Pure astrocyte cultures (n =11–12) were treated with actinomycin D (ACT D; 12.5 μg/ml) or cycloheximide (CHX; 1 μg/ml) in the absence (white bars) or presence (black bars) of IL-1β (3 ng/ml) for 20–24 hr following which ¹⁴C-L-cystine uptake (3 μM labeled + 27μM unlabeled; 25°C) was determined. Data are expressed as mean ± SEM ¹⁴C-L-cystine uptake in pmol/30 min/mg protein. An asterisk (*) denotes values different from control (-IL-1β) and a pound sign (#) indicates values different from IL-1β-treated conditions as assessed by two-way ANOVA followed by Bonferroni’s post hoc test. Significance was set at p < 0.05.