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Cumulative percent changes in frequency of breathing (top panel), tidal volume (middle panel) and minute ventilation (bottom panel) elicited by morphine (10 mg/kg, i.v.) in conscious rats pretreated with vehicle (VEH) or naloxone (NLX, 1.5 mg/kg, i.v.) on Day 1, and again on Day 2 in these rats. Data are mean ± SEM. There were six rats in each group. *P < 0.05, significant cumulative response. †P < 0.05, NLX-treated versus vehicle-treated rats. ‡P < 0.05, Day 2 versus Day 1.
