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. 2015 Jun 2;6:391. doi: 10.3389/fpls.2015.00391

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Copyright © 2015 Lu, Wei and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isolation of GCs from just-germinated pollen grains. (A) Pollen grains were geminated in vitro for 20 min, and most produced pollen tubes. (B) 10-min osmotic shock of germinated pollen grains in osmotic shock solution led to a burst of most of pollen tubes. The inset image in (C) and (D) shows change of just-released GC from spindle-shaped (C) to oval-shaped (D). (E,F) DIC microscopy (E) and viability with fluorescein diacetate (FDA) staining (F) of purified GCs. Close-up of GCs in inset images (E,F). Scale bar: 20 μm.