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. 2015 Jun 2;6:391. doi: 10.3389/fpls.2015.00391

FIGURE 4.

FIGURE 4

Isolation of SCs from 10-h–cultured pollen tubes. (A) DIC (upper) and DAPI staining (middle) and merged (bottom) images of pollen tube. (B) Representative enzymolysis-treated pollen tubes. (C,D) DIC microscopy (C) and viability of purified SCs with FDA staining (D). The inset images in (C) and (D) are close-ups of the SC. Scale bar: 20 μm in (A) to (D), 10 μm in inset images of (C) and (D).