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. 2015 Jun 2;6:391. doi: 10.3389/fpls.2015.00391

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Copyright © 2015 Lu, Wei and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isolation of VN from pollen tubes. (A,B) DIC (A) and DAPI staining (B) of pollen tubes cultured in vitro for 1.5 h. (C,D) DIC microscopy (C) and propodium iodide (PI) staining (D) of enzymolysis-treated pollen tubes; arrows indicate released VNs (D). (E,F) DIC microscopy (E) and PI staining (F) of purified VN. Inset image in (F) is close-up of a PI-stained VN. Scale bar: 20 μm in (A) to (F), 10 μm in the inset image (F).