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. 2015 Jun 2;6:165. doi: 10.3389/fphys.2015.00165

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Copyright © 2015 Tamaki, Uchiyama, Hirata, Hashimoto, Nakajima, Saito, Terachi and Mochida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of CD markers in freshly isolated and expanded (passage 1) Sk-Cs. (A) Freshly isolated total cells were positive for 6 out of 11 markers, and predominant markers were CD29 and CD34; thus, these were used for subsequent cell fractionation. However, expression of these markers markedly changed after culture of both Sk-DN/29⁺ and Sk-34/29^+/− cells, but importantly, the characteristics of both cells were similar after expansion. (B) Actual sorting for the exclusion of CD45⁺ cells, as hematopoietic cells. (C) Basic sorting pattern of 3 cell fractions, as Sk-DN/29⁺, Sk-34/29⁺ and Sk-34/29⁻, which was consistently used throughout the present study. Data were obtained from 21 subjects/32 samples (Male n = 19, age 21–79; Female n = 2, age 17–69, from abdominal muscle n = 16, leg muscles n = 5).