Table 1.

Optimal culture conditions for human Sk-34 and Sk-DN cells.

	Awaking medium	Proliferation medium (from passage 1)
	Common use	Sk-34/29+/−fraction	Sk-DN/29+ fraction
Basic contents	IMDM	IMDM	IMDM
	20%FBS	20%FBS	20%FBS
	0.1 mM 2-Mercaptethanol	0.1 mM 2-Mercaptethanol	0.1 mM 2-Mercaptethanol
	50 μg/ml gentamicin	50 μg/ml gentamicin	50 μg/ml gentamicin
Additional contents	0.4 ng/ml EGF	0.4 ng/ml EGF	0.4 ng/ml EGF
	12.5 ng/ml bFGF	12.5 ng/ml bFGF	12.5 ng/ml bFGF
	20 ng/ml IGF-1	20 ng/ml IGF-1
	4 mg/ml Dexamethasone