Figure 5 –

sPLA₂ V stimulates MUC5AC, LTB₄, and LTC₄ secretion from goblet cells, but not ciliated cells. A and B, Apical and basolateral sPLA₂ V increased MUC5AC secretion from goblet-enriched cell culture. MUC5AC protein was measured in supernatants from ciliated or goblet cells. A, Apical or basal 60-min exposure to sPLA₂ V at 0, 0.02, 0.1, 0.5, or 1 μg/mL. Data are presented as mean ± SEM from five samples. #P < .001 for ciliated cells, *P < .05, **P < .02, ***P < .01 for goblet cells compared with PBS, ^§P < .02, ^§§P < .01, ^§§§P < .001. B, Apical or basal 15-, 30-, or 60-min exposure to sPLA₂ V (0 or 0.5 μg/mL). Data are presented as mean ± SEM from five samples. *P < .05, **P < .01. C and D, Apical sPLA₂ V stimulates LTB₄ and LTC₄ secretion from goblet and ciliated-enriched cells. Secreted LTB₄ or LTC₄ in supernatants of ciliated-enriched and goblet-enriched cells stimulated for 24 h with apical sPLA₂ (0, 0.005, 0.05, or 0.5 μg/mL). C, LTB₄. D, LTC₄. Data are presented as mean ± SEM from four samples. *P < .01. See Figure 1 and 3 legends for expansion of abbreviations.