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. Author manuscript; available in PMC: 2015 Jun 2.
Published in final edited form as: J Biol Chem. 1990 May 15;265(14):8190–8197.

Fig. 4. Protein sequencing of 7α-hydroxylase.

Fig. 4

A Coomassie Blue-stained gel of 20 µg of step 6-purified material (Table I) is shown together with the amino-terminal sequences determined from each of the four major proteins remaining at this stage of the purification. The calculated molecular weight of each protein is shown on the left. The identities of cytochrome P-450a and cytochrome P-450g were determined by comparison of their deduced amino-terminal sequences with those in the National Biomedical Research Foundation protein data base and by enzyme assay (see “Results”).