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Expression of 7α-hydroxylase in transfected COS-M6 cells
| Experiment | Plasmid | Microsomal protein |
Cytochrome P-450 reductase |
Conversiona | Specific activity |
|---|---|---|---|---|---|
| µg | % | Pmol/min/mg protein | |||
| 1 | Vector alone | 250 | + | <0.1 | <5.0 |
| 500 | + | <0.1 | <2.5 | ||
| 7α-Hydroxylase cDNA | 250 | + | 2.5 | 130 | |
| 500 | + | 4.9 | 127 | ||
| 2 | Vector alone | 250 | + | <0.1 | <5.0 |
| 500 | + | <0.1 | <2.5 | ||
| 7α-Hydroxylase cDNA | 250 | + | 3.7 | 192 | |
| 500 | − | 0.4 | 10 | ||
| 500 | + | 7.2 | 187 |
% conversion of [14C]cholesterol (25 µM) into 7α-hydroxycholesterol in 20 min.
