Table 1.
M. truncatula , C. arietinum , G. max , L. angustifolius T2-RNases larger than 500 bp, that encode putative proteins not presenting in their amino acid sequence amino acid pattern 4 according to Vieira, et al. [28]
| Locus | Gene code | IP | Intron number | Motif 1 | Motif 2 | Motif 4 | Location |
|---|---|---|---|---|---|---|---|
| T. pratense | |||||||
| [GenBank:ASHM01010303] { | Tp1 | 9.20 | 1 | FVIHGLWPSR | WPSLKYN | - | ASHM01010303: 956… 1742 |
| [GenBank:ASHM01021082] | Tp2 | 8.82 | 1 | FTIHGMWPSN | WPSYTSP | - | ASHM01021082: 467… 1277 |
| [GenBank:ASHM01011821] | Tp3 | 7.57 | 1 | FSVHGVWPTN | WPDLKGG | - | ASHM01011821: 2194… 2920 |
| [GenBank:ASHM01032414] | Tp4 | 9.20 | 1 | FVIHGLWPVF | WPSLKYN | - | ASHM01032414: 1330… 2116 |
| [GenBank:ASHM01032369 ]+ | Tp5 | 9.92 | 1 | FTIHGLWPSN | WPNLKWT | - | ASHM01032369: 1121… 2019 |
| [GenBank:ASHM01005450] | Tp6 | 9.18 | 1 | FSLHGLWPSN | WPSLFVG | - | ASHM01005450: 3673… 4373 |
| [GenBank:ASHM01035891] | Tp7 | 9.06 | 1 | FTIHGLWPSN | WPNLLMV | - | ASHM01035891: 1083… 2003 |
| [GenBank:ASHM01035915] | Tp8 | 9.51 | 1 | FTLHGIWPSN | WPDLKGQ | - | ASHM01035915: 1152… 2109 |
| [GenBank:ASHM01087496] | Tp9 | 8.11 | 1 | FSIHGLWPQN | WPSLTGN | - | ASHM01087496: 1… 681 |
| [GenBank:ASHM01016923] | Tp10 | 6.87 | 1 | FSIHGLWPQN | WPSLTGK | - | ASHM01016923: 1540… 2300 |
| [GenBank:ASHM01047800] | Tp11 | 9.48 | 1 | FTTHGLWPSN | WPNLKGP | - | ASHM01047800:1… 629 |
| [GenBank:ASHM01027928] | Tp12 | 8.75 | 1 | FTIHGLWPSN | WPNLLSN | - | ASHM01027928:226… 1002 |
| [GenBank:ASHM01008805] | Tp13 | 8.85 | 1 | FSIHGLWPQN | WPSLTGN | - | ASHM01008805:250… 977 |
| [GenBank:ASHM01049573] | Tp14 | 8.64 | 1 | FTTHGLWPSN | WPNLKGP | - | ASHM01049573:1… 575 |
| [GenBank:ASHM01036061] { | Tp15 | 9.20 | 1 | FVIHGLWPSI | WPSLKYN | - | ASHM01036061: 956… 1742 |
| M. truncatula | |||||||
| [GenBank:AC123571.8 (Medtr5g022810)] | Mt1 | 7.57 | 2 | FVMHGLWPAN | WPDLLVY | - | Mt5:8,780,338..8,781,194 |
| [GenBank:AC149207.1 (Medtr2g021830)] | Mt2 | 7.06 | 1 | FTLHGLWPSN | WPNLFGA | - | Mt2:7,405,970..7,406,697 |
| [GenBank:AC149207.2] | Mt3 | 8.57 | 2 | FTVHGLWPSN | WPSVTTT | - | Mt2:7,383,161..7,384,370 |
| [GenBank:AC149269.11 (Medtr6g090200)] | Mt4 | 6.39 | 1 | FTIQGLFPNN | WINYIGD | - | Mt6:22,040,215..22,039,455 |
| [GenBank:AC159124.1] > | Mt5 | 9.06 | 2 | LTVHGLWPSN | WPDVGGT | - | Mt2:7,374,496..7,375,004 |
| [GenBank:AC196855-3 (Medtr2g104330)] { | Mt8 | 8.05 | 1 | FTLHGFWPSN | YPFDFNT | DFNTTK | Mt2:34,011,354..34,010,761 |
| [GenBank:CR936945 (Medtr5g086410)] | Mt9 | 8.45 | 1 | LTIRGLWPST | WPSLNSG | - | Mt5:36,330,498..36,331,243 |
| [GenBank:CU459033 (Medtr5g086770)] | Mt10 | 5.78 | 2 | FKIWGLWPVR | WPSLFGP | SLFGPD | Mt5:36,498,402..36,499,282 |
| [GenBank:CT573354] | Mt12 | 8.83 | 1 | FTIHGVWPSN | WPRLDTA | - | Mt3:9,158,726..9,157,789 |
| [GenBank:CU026495] | Mt13 | 8.83 | 1 | FTIHGLWPSN | WPRLDTA | - | Mt3:9,139,338..9,138,417 |
| [GenBank:AC126012 (Medtr5g0977101)] | Mt14 | 5.20 | 1 | FLLYGAWPVD | WRDIKNG | IKNGDD | Mt5:41,755,316..41,755,711 |
| [GenBank:AC233685_48.1 (XM003637773)] | Mt16 | 9.21 | 1 | FTIHGLWPTN | WPDVIHG | - | MtU:12,302,642..12,303,437 |
| [GenBank:Medtr2g021910.2] | Mt17 | 8.54 | 2 | LTIHGLWPSN | WPSIYGD | IYGDDD | Mt2:7,440,534..7,441,199 |
| Mettr2g021910.2 | Mt18 | 8.40 | 2 | LTIHGLWPSN | WPTIYGS | IYGSDD | Mt2:7,445,004..7,445,674 |
| [GenBank:AC124218 (XM003624084)] | Mt20 | 8.82 | 1 | FTIHGLWVEN | WPSLYQK | LYQKSS | Mt7:22,479,456..22,480,238 |
| [GenBank:CM001222 { | Mt23 | 9.55 | 1 | FSIHGLWPTN | WPDAVYG | - | Mt6:12,596,544..12,596,922 |
| BT148419] | Mt24 | 5.77 | 1 | FTIHGLWPDY | WPSLSCG | - | MtT:10,244,733..10,244,880 |
| [GenBank:BT136026 (AFK35821)] | Mt25 | 6.86 | 3 | FTFILQWPGS | WPSLRCP | CPRLNN | Mt5:17,636,584..17,636,691 |
| [GenBank:AW776643] > | Mt26 | 8.47 | n.a | FGIHGLWPTN | WPNLLEW | - | - |
| C. arietinum | |||||||
| [GenBank:XP_004503396 (NC021165)] | Ca1 | 8.62 | 1 | FTIHGLWPSN | WPNLKGQ | - | Ca6:2,486,865..2,487,892 |
| [GenBank:CM001766.1] | Ca2 | 9.35 | 1 | LTVHILWGTN | WNDHSFC | - | Ca3:9,734,288..9,735,009 |
| [GenBank:XP_004486305 (CM001764.1)] { | Ca3 | 9.44 | 1 | FTVHGLWPSN | WPNLFGN | - | Ca1:34,647,053..34,647,728 |
| [GenBank:XP_004486305 (CM001764.1)] | Ca4 | 7.59 | 1 | FTVHGLWPSN | WPNLFGN | - | Ca1:5,252,166..5,252,821 |
| [GenBank:CM001767.4] | Ca5 | 8.47 | 1 | FTIHGLWPYN | WPDLKGQ | - | Ca4:42,156,653..42,157,571 |
| [GenBank:CM001767.3] { | Ca6 | 6.43 | 2 | FIIHGLWPSN | WPNLKGQ | - | Ca4:3,880,028..3,880,839 |
| [GenBank:CM001768.1] { | Ca7 | 9.02 | 1 | FTIHGLWPSN | WSNLKGQ | - | Ca5:12,546,395..12,547,197 |
| [GenBank:CM001768.2] | Ca8 | 9.24 | 1 | FTIHGLWPFN | WPNLNGQ | - | Ca5:11,753,113..11,753,945 |
| [GenBank:CM001769.1] | Ca9 | 9.28 | 1 | FTIHGLWPNN | WPSLIKG | - | Ca6:45,431,765..45,432,712 |
| [GenBank:XP_004505385 (CM001769.2)] | Ca10 | 8.85 | 1 | FTIHGLWPSN | WPNLKGQ | - | Ca6:16,977,346..16,978,256 |
| [GenBank:CM001769.4] | Ca11 | 8.61 | 1 | FTLHGLWPSN | WPNLNGV | - | Ca6:31,097,283..31,098,019 |
| [GenBank:CM001769.5] { | Ca12 | 7.79 | 1 | FTIHGLWPSN | WPSLTMS | - | Ca6:28,777,475..28,778,149 |
| [GenBank:CM001769.6] { | Ca13 | 9.03 | 1 | FTLHGLWPSN | WPNLNGG | - | Ca6:33,284,148..33,284,935 |
| [GenBank:CM001769.7] > | Ca14 | 8.27 | 1 | KIIHGLWPSN | PSLTKSQ | - | Ca6:28,744,494..28,745,117 |
| [GenBank:CM001769.8] | Ca15 | 8.80 | 1 | FTIHGLWPSN | WPNLKGQ | - | Ca6:2,486,787..2,487,895 |
| [GenBank:XP_004507007 (CM001769.9)] | Ca16 | 9.17 | 1 | FTIHGLWGTN | WPDVINQ | - | Ca6:52,088,714..52,089,462 |
| [GenBank:XP_004503396 (CM001769.10)] | Ca17 | 9.09 | 1 | FTIHGLWPSN | WPNLKGQ | - | Ca6:2,486,751..2,487,895 |
| [GenBank:XP_004505385 (CM001769.11)] | Ca18 | 8.85 | 1 | FTIHGLWPSN | WPNLKGQ | - | Ca6:16,977,346..16,978,256 |
| [GenBank:XP_004514375 (gi484567706)] > | Ca19 | 8.47 | 1 | FTLHGLWPSN | WPNLNGV | - | scaffold485:91,749..192,162 |
| [GenBank:XP_004506021 (gi484571392)] | Ca20 | 9.02 | 1 | FKIHGLWPSN | WPSLIDS | - | Ca6:28,325,256..28,326,148 |
| [GenBank:XP_004515186 (gi484566269)] | Ca21 | 9.16 | 1 | FKIHGLWPNT | WPSLKKS | - | scaffold948:113,466..114,365 |
| G. max | |||||||
| [GenBank:CM000836] | Gm1 | 9.05 | 1 | FTIHGLWPQN | WPNLNTQ | - | GM03: 42522935… 42523824 |
| [GenBank:XP_003548020)] { | Gm2 | 5.71 | 2 | FTISYFRPRK | WPDLTTD | - | GM16: 30294108… 30295346 |
| [GenBank:NP_001235172] | Gm3 | 6.80 | 2 | FTISYLHPMR | WPDLRTD | - | GM02: 5707162… 5708520 |
| [GenBank:XP_003519927] | Gm4 | 5.47 | 2 | FTISYFRPRK | WPDLRTD | - | GM02: 5686955… 5688178 |
| [GenBank:XP0035181161] | Gm5 | 7.49 | 2 | FTISYLHPMR | WPDLRTD | - | GM02: 5682344… 5683625 |
| [GenBank:XP003518119] | Gm6 | 6.30 | 2 | FTISYLHPMR | WPDLRTD | - | GM02: 5698841… 5700244 |
| [GenBank:CM000853] | Gm7 | 8.61 | 3 | FSIHGLWPNF | WASLSCA | - | GM20:5212321… 5214271 |
| L. angustifolius | |||||||
| [GenBank:AOCW01152977] | La1 | 9.04 | 0 | FTLHGLWPIN | WPNLNGK | - | scaffold92513_2 |
IP- isoelectric point.
Underscored are amino acids that are not allowed in the motifs of [28].
+ sequences presenting stop codons in the putative coding region.
{ sequences where gaps were introduced to avoid stop codons in the putative coding region.
> very divergent sequences that, although they present all the criteria of S-lineage S-RNase genes, were not included in phylogenetic analyses.