. 2015 Jun 3;22(1):38. doi: 10.1186/s12929-015-0139-x

Table 1.

The IC50 of sulforaphane for human NSCLC cells harboring different EGFR mutations

Cell line	EGFR mutation status	Sulforaphane
Cell line	EGFR mutation status	IC50 (μM) ^a
A549	Wild-type	10.2 ± 0.15
CL1-5	Wild-type	9.7 ± 0.33
H3255	L858R ^b	14.5 ± 0.21
PC9/gef ^c	Exon 19 del.	7.3 ± 0.25
H1975	L858R/T790M ^d	5.9 ± 0.18
HFB	Wild-type	65.4 ± 0.29

^aCells were treated with various concentrations of sulforaphane for 48 h, and cell viability was determined by MTT assay. The IC50 values were calculated by non-linear regression analysis. Values are given as means ± standard deviation

^bL858R substitution in exon 21

^cPC9/gef is a gefitinib-resistant cell line that was selected from parental PC9 cells (which contain a deletion in exon 19 of EGFR) by continuous exposure to increasing doses of gefitinib over ~ 6 months

^dL858R substitution in exon 21 and a secondary T790M substitution in exon 20 of EGFR