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. 2015 Jun 3;14:74. doi: 10.1186/s12934-015-0263-z

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© Gratz et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Outer membrane preparation of CK2-presenting E. coli. Outer membrane samples were separated by SDS-PAGE (7.5% acrylamide) and stained with Coomassie brilliant blue. For autodisplay of the individual CK2 subunits, E. coli BL21(DE3) pCK2α (lanes 5 and 6) or E. coli BL21(DE3) pCK2β (lanes 7 and 8) was used, for autodisplay of both subunits in one strain E. coli BL21(DE3) pCK2α pCK2β (lanes 3 and 4) was used. A fraction of each whole cell sample was treated with trypsin before preparation of outer membranes to degrade surface presented proteins facing toward the extracellular medium (lanes 2, 4, 6 and 8). The host strain without any autodisplay construct served as a control (lane 1 and 2).