Table 1. Primer and probe sequences used in identification of Borrelia bavariensis in 11-year-old patient with oligoarthritis, Vienna, Austria, February 2014*.
Primer or probe | Target gene | Sequence, 5′ → 3′ | Reference |
---|---|---|---|
Primer | |||
16SF | 16S rDNA | GCT GTA AAC GAT GCA CAC TTG GT | (6) |
16SR | 16S rDNA | GGC GGC ACA CTT AAC ACG TTA G | (6) |
BorF | Flagellin | GAA TTA GCA GTT CAA TCA GG | (7) |
BorR | Flagellin | TTC GTC TGT AAG TTG CTC TAT | (7) |
rrf-rrl IGS F | 5S–23S IGS | CTG CGA GTT CGC GGG AGA | (8) |
rrf-rrl IGS R | 5S–23S IGS | TCC TAG GCA TTC ACC ATA | (8) |
B5S-23S_Fn | 5S–23S IGS | GAG TTC GCG GGA GAG TAA G | (8) |
B5S-23S_Rn | 5S–23S IGS | TAG GCA TTC ACC ATA GAC TCT T | (8) |
V1a | ospA | GGG AAT AGG TCT AAT ATT AGC | (10) |
V1b | ospA | GGG GAT AGG TCT AAT ATT AGC | (10) |
V3a | ospA | GCC TTA ATA GCA TGT AAG C | (10) |
V3b | ospA | GCC TTA ATA GCA TGC AAG C | (10) |
R1 | ospA | CAT AAA TTC TCC TTA TTT TAA AGC | (10) |
R37 | ospA | CCT TAT TTT AAA GCG GC | (10) |
Probe | |||
LBTM | 16S rDNA gene | FAM–TTC GGT ACT AAC TTT TAG TTA A–TAMRA | (6) |
BorTM | Flagellin gene | FAM–AAC GGC ACA TAT TCA GAT GCA GAC–TAMRA | (7) |
*IGS, intergenic spacer.