Skip to main content
. 2015 Jun;21(6):1052–1054. doi: 10.3201/eid2106.141516

Table 1. Primer and probe sequences used in identification of Borrelia bavariensis in 11-year-old patient with oligoarthritis, Vienna, Austria, February 2014*.

Primer or probe Target gene Sequence, 5′ → 3′ Reference
Primer
16SF 16S rDNA GCT GTA AAC GAT GCA CAC TTG GT (6)
16SR 16S rDNA GGC GGC ACA CTT AAC ACG TTA G (6)
BorF Flagellin GAA TTA GCA GTT CAA TCA GG (7)
BorR Flagellin TTC GTC TGT AAG TTG CTC TAT (7)
rrf-rrl IGS F 5S–23S IGS CTG CGA GTT CGC GGG AGA (8)
rrf-rrl IGS R 5S–23S IGS TCC TAG GCA TTC ACC ATA (8)
B5S-23S_Fn 5S–23S IGS GAG TTC GCG GGA GAG TAA G (8)
B5S-23S_Rn 5S–23S IGS TAG GCA TTC ACC ATA GAC TCT T (8)
V1a ospA GGG AAT AGG TCT AAT ATT AGC (10)
V1b ospA GGG GAT AGG TCT AAT ATT AGC (10)
V3a ospA GCC TTA ATA GCA TGT AAG C (10)
V3b ospA GCC TTA ATA GCA TGC AAG C (10)
R1 ospA CAT AAA TTC TCC TTA TTT TAA AGC (10)
R37 ospA CCT TAT TTT AAA GCG GC (10)
Probe
LBTM 16S rDNA gene FAM–TTC GGT ACT AAC TTT TAG TTA A–TAMRA (6)
BorTM Flagellin gene FAM–AAC GGC ACA TAT TCA GAT GCA GAC–TAMRA (7)

*IGS, intergenic spacer.