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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2015 Mar 5;30:1–10. doi: 10.1016/j.dnarep.2015.02.021

Fig. 2.

Fig. 2

Schematic representation of the BER enzymes digestion assay for visualization of AlkB-mediated repair of ε-lesions.32 P-labeled ssDNA ordsDNA oligodeoxynucleotides containing single ε-lesion are incubated with AlkB protein. Subsequently, to visualize the AlkB-mediated repair, oligodeoxynucleotides are treated with the appropriate DNA glycosylase (G) followed by AP endonuclease 1 (AP). Reaction products are separated by denaturing PAGE and visualized by phosporimaging. The dashed line indicates the complementary, lesion-free unlabeled oligodeoxynucleotide, which is either present during the repair reaction (dsDNA repair) or is added post-repair (ssDNA repair). G/AP cleavage of the DNA substrate occurs only if the ε-lesion is not removed. A repaired substrate will appear as a slower-migrating band on PAGE.