Table 7.
Plant organ ( Arabidopsis ) | Experiments (replicates) | Database size (# sequences) | Hits (%) | Hits with array elements that are not expressed in the specific experiments (%) |
---|---|---|---|---|
Flower - not pollinated | 21 (54) | 20,032 | 11,970 (59.8) | 331 (2.7) |
Flower - pollinated | 16 (40) | 19,406 | 11,844 (61.0) | 457 (3.7) |
Flower - overall | 37 (94) | 20,372 | 12,035 (59.1) | 266 (2.2) |
Seed | 50 (98) | 20,679 | 12,003 (58.0) | 298 (2.4) |
Embryo | 6 (12) | 17,275 | 10,995 (63.6) | 1,306 (10.6) |
Endosperm | 16 (34) | 19,247 | 11,451 (59.5) | 850 (6.9) |
The transcriptome coverage was estimated by comparing annotated Hypericum sequences with publically available Arabidopsis microarray data (http://www.ebi.ac.uk/arrayexpress/). For each plant component, such as flower and seed, the number of microarray experiments and replicates that were used to estimate the size of each database are reported. The recovered hits indicate the number (and percentage) of database sequences that matched Hypericum accessions. For each plant component, the number of array elements that are not expressed in the specific experiments (non-significant hybridization signals), but that produced significant matches with Hypericum sequences is also reported.