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. 2015 May 19;11(5):e1005194. doi: 10.1371/journal.pgen.1005194

Fig 5. Effect of the impairment of the piRNA and miRNA pathways on the accumulation of four individual piRNAs.

Fig 5

Analysis by RT-qPCR of the changes in the steady-state levels of four major piRNAs originating from ZAM and Tabor (two follicle cell-specific TEs) and from the 3' UTR of the traffic jam (tj) and jim genes (see sequences in S3 Table), upon AGO1 soma KD (tj-GAL4>shAGO1), expression of the trans-dominant negative Drosha construct (tj-GAL4>TN-Drosha), or piwi soma KD (tj-GAL4>shpiwi). Quantification was done relative to the 42AB piRNA (a germline-specific piRNA: S3 Table) and normalized to the respective controls (tj-GAL4>shAGO3, tj-GAL4>Ø and tj-GAL4>shAGO3) (bars represent the mean ± SD of ≥ three biological replicates). P-values were evaluated by two-tailed Student’s t-tests (for ZAM and Tabor, all P-values were lower than 0.007).