. 2014 Nov 19;2:36. doi: 10.1186/s40591-014-0036-9

Table 1.

Summary of effects of small-molecule antagonists of the Tcf/β-catenin complex on endometriosis

Model	Cell type or species	Inhibitor	Parameters assessed	Functional effects	Reference
In vitro assay	C-EE, C-ES	PKF 115-584	Cell proliferation	C-EE, C-ES, E-EE, E-ES, EnE and EnS: significantly inhibited vs. non-treated cells	[4]
	E-EE, E-ES	CGP049090
	EnE, EnS	CGP049090
	C-EE, C-ES	PKF 115-584	Cell migration	C-EE, C-ES, E-EE, E-ES, EnE and EnS: significantly inhibited vs. non-treated cells	[4]
	E-EE, E-ES
	EnE, EnS
	C-EE, C-ES	PKF 115-584	Cell invasion	C-EE, C-ES, E-EE, E-ES, EnE and EnS: significantly inhibited vs. non treated cells	[4]
	E-EE, E-ES
	EnE, EnS
	C-EE, C-ES	PKF 115-584	Tcf/β-catenin target genes (Cyclin D1, Survivin, c-Myc, MMP2, MMP9)	C-EE, C-ES, E-EE, E-ES, EnE and EnS: cyclin D1, Survivin, MMP2 and MMP9 mRNA: significantly decreased vs. non-treated cells	[4]
	E-EE, E-ES
	EnE, EnS
				Total form of MMP-9 in E-EE or E-ES: significantly decreased vs. C-EE or C-ES
				Active forms of MMP-2 in EnE: significantly decreased vs. E-EE
				Total form of MMP-9 in EnS: significantly decreased vs. E-ES
	E-ES, EnS	PKF 115-584	Fibrotic markers (αSMA, Col-I, CTGF, and FN)	E-ES, EnS: αSMA, Col-I, CTGF, and FN mRNA (with or without TGF ß1 stimulation): significantly decreased vs. non-treated cells	[4]
	E-ES, EnS	CGP049090	Fibrotic markers (αSMA, Col-I, CTGF, and FN)	EnS: percentage of αSMA-positive cells: significantly decrased vs. non-treated cells
	E-ES, EnS	PKF 115-584	Collagen gel contraction	E-ES, EnS: significantly decreased vs. non-treated cells	[5]
	E-ES, EnS	CGP049090	Collagen gel contraction	E-ES, EnS: significantly decreased vs. non-treated cells	[5]
In vivo assay	Mouse (female Swiss nude mice)	CGP049090	Severity of fibrosis in endometriotic implants assessed by Sirius Red or Masson Trichrome stains	Staining scores for Sirius Red or Masson Trichrome stains: significantly lower vs. non-treated mice	[5]

C-EE: endometrial epithelial cells of patients without endometriosis, C-ES: endometrial stromal cells of patients without endometriosis.

E-EE: Endometrial epithelial cell of patients with endometriosis, E-ES: endometrial stromal cell of patients with endometriosis.

EnE: Endometriotic epithelial cells, EnS: endometriotic stromal cells.

MMP-2: Matrix metalloproteinase-2, MMP-9: Matrix metalloproteinase-9, αSMA: alpha smooth muscle actin, Col-I: Type I collagen.

CTGF: connective tissue growth factor, FN: fibronection.