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. 2014 Feb 1;2:3. doi: 10.1186/2052-8426-2-3

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© Zheng et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Real-time dynamic observation of cultured lung telocytes through cell IQ cell culturing platform. The granular material (black arrow) appeared within the cellular frame, attracted cytoplasma, and was rounded with rich cytoplasma (A-D). Images were captured at 20 min intervals. Magnification is 200×. HPMECs were divided into two groups and cultured for 72 hours with DMEM and TCM respectively, and the proliferation of the cell was detected at 0 h, 12 h, 24 h, 48 h and 72 h. As showed in the figure, the proliferation of HPMECs cultured with TCM was faster than that of DMEM (P < 0.05).