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. 2015 May 18;2015:391043. doi: 10.1155/2015/391043

Figure 8.

Figure 8

IWR-1 inhibits WNT3a-stimulated β-catenin/TCF signaling activity in embryonic CD24+ cells from E15 mouse kidney. CD24+ cells from mice bearing the β-catenin/TCF reporter transgene were isolated by FACS from E15 mouse kidneys and cultured for 12 hours. IWR-1 was then added and CD24+ cells were exposed to L-cells expressing WNT3a for 12 hours to assess β-catenin/TCF signaling activity. (a) β-galactosidase activity per μg cell protein was maximally inhibited to 34% of control by IWR-1 concentrations 100 μM (24 hours). (b) CD24+ cells were exposed to control medium (black bars) or IWR-1 (100 μM) (white bars) for 12 hours and then transferred to control medium for various periods of time to track persistence of WNT3a-stimulated β-galactosidase activity; significant inhibition was evident after 12 hours (36% of control) and 72 hours (43% of control) of washout in control medium.