Figure 1.

The rat corpus callosum (CC). (a) Sagittal sections from young female rat brains were immunostained for GFAP, and nuclei were labeled with DAPI. The CC was divided into three regions: genu (G), body (B), and splenium (S) to determine glial cell density. Other brain regions are indicated for anatomical reference: caudate-putamen (CPu), hippocampus (Hip), and lateral ventricle (LV). Arrows show anatomical orientation: dorsal (D), ventral (V), anterior (A), and posterior (P). (b) Higher magnification of the caudal body of the rat CC; notice that the lateral ventricle (LV) contains the choroid plexus (ChP) in close interaction with GFAP-positive cells from the CC. (c) Higher magnification of the square in (b) to highlight fibrous astrocytes from the body of the CC; notice that nuclei stained by DAPI show a radial orientation, and astrocytes align accordingly (arrowheads). Scale bars: 200 μm (a), 100 μm (b), and 50 μm.