Skip to main content
. Author manuscript; available in PMC: 2015 Jun 2.
Published in final edited form as: Ann Biomed Eng. 2009 Jun 10;37(8):1613–1628. doi: 10.1007/s10439-009-9726-x

FIGURE 11.

FIGURE 11

Next-image processing of cryo-images containing individual stem cells. A cryo-image of fluorescently labeled cells at a fracture site in a mouse before (a) and after a single 40 μm section was taken (b). Images were taken of a parabiosis mouse model in which a GFP positive transgenic mouse and a GFP negative littermate had surgically joined circulatory system. Performing next image processing with μ = 214 cm−1 and σ = 9 μm on (a) out-of-plane fluorescence was removed (c). Out-of-plane fluorescence was successfully removed leaving in-plane fluorescence from two surface GFP positive cells.