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. 2015 Apr 1;106(5):550–558. doi: 10.1111/cas.12654

Fig 4.

Fig 4

ERAP regulates signaling pathways by the crosstalk of E2 and a variety of growth factors. (a) An MTT assay showing the inhibitory effect of ERAP on the growth of BT-474 cells by E2 and/or EGF stimulation. These data represent the mean ± SD of three independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001). (b) The inhibitory effects of ERAP on Akt, MAPK, and estrogen receptor alpha (ERα) phosphorylation induced by E2 and/or EGF stimulation in BT-474 cells. The data are expressed as the fold increase over untreated cells at 0 h. (c) Inhibitory effects of ERAP on the interactions of estrogen receptor alpha (ERα) with human epidermal growth factor 2 (HER2), epidermal growth factor receptor (EGFR), insulin-like growth factor 1 receptor beta (IGF-1Rβ) or PI3K. (d, e) The inhibitory effects of ERAP on the interactions of EGFR (d) or HER2 (e) with ERα and Shc in BT-474 cells. The above all blot were cropped, and the full-length blots are included in Supplementary Figure S4. (f) Schematic illustrations of inhibitory effects of ERAP on cell functions caused by the crosstalk of ERα and IGF-1R, EGFR or HER2.