Fig 3.

Western blot analysis of two transiently transfected prostate cancer cell lines, Panc-1 and MIAPaCa-2. Akt, protein kinase B; COMT, catechol-O-methyltransferase; COMT-OE, COMT protein overexpression cell lines; COMT-miR, miRNA-interfering COMT cell line; Control-OE, normal COMT protein expression; Control-miR Control-COMT cell line, without miRNA-interference of COMT expression; GSK3, glycogen synthase kinase 3; MB-COMT, membrane-binding COMT; PI3K, phosphatidylinositol 3-kinase; PTEN, phosphatase and tensin homolog.