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. 2015 Mar 25;106(5):576–583. doi: 10.1111/cas.12648

Fig 4.

Fig 4

Catechol-O-methyltransferase (COMT) downregulation promotes cell growth, increases the S-phase cell ratio, reduces apoptosis, and promotes invasion in vitro. (a) Western blot for COMT proteins for transduction. (b) Average ratio of normalized membrane-binding COMT (MB-COMT) expression. *P < 0.0001. COMT-miR, cell lines stably transfected with siRNA; Control-miR, normal COMT protein expression. (c) Average ratio of normalized soluble COMT (S-COMT) expression. *P < 0.0001. (d) Relative proliferation at 24, 48, 72, and 96 h. *P < 0.05. (e) Flow cytometry assay for the cell cycle. (f) Relative number of cells in S phase. *P < 0.05. (g) Flow cytometry assay for apoptosis. (h) Relative cell number of the apoptosis rate. *P < 0.001. (i) Transwell assay for cell invasion. (j) Migrated cells per field. *P < 0.05. (k) Western blotting for molecules critical for cell proliferation, apoptosis, and invasion. Akt, protein kinase B; GSK3, glycogen synthase kinase 3; PI3K, phosphatidylinositol 3-kinase; PTEN, phosphatase and tensin homolog. (b–d, f, h, j) Error bars indicate SD.