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. 2015 Mar 26;106(5):656–664. doi: 10.1111/cas.12649

Fig 5.

Fig 5

Effect of neural epidermal growth factor-like like 1 (NELL1) and NELL2 on renal cell carcinoma cell migration and adhesion. (a–d) Transfilter cell migration assay. The bottom side of the Transwell filter was coated with 40 nM or 400 nM alkaline phosphatase (AP), NELL1-AP or NELL2-AP. OS-RC-2 (a) or VMRC-RCW (b) cells were placed inside the Transwell chamber and allowed to migrate to the bottom side of the filter overnight. The cells that migrated to the bottom side of the filter were stained with hematoxylin and photographed. (c, d) Quantification of cell migration. The numbers of cells that migrated to the bottom side of the filter were plotted as mean ± SD. (e–l) Cell adhesion assay. OS-RC-2 (left three wells of e, and f, h, j) and VMRC-RCW (right three wells of e, and g, i, k) cells were seeded on AP (e, f, g), NELL1-AP (e, h, i), or NELL2-AP (e, j, k) coated dishes (400 nM each). Low (e) and high (f–k) power views after cell adhesion assay. Adherent cells were stained with hematoxylin. Adherent cell numbers were quantified and plotted as means ± SD (l). HPF, high-power field.