Table 3. Kinetic parameters of KPC variants.
AMP | IMI | MERO | CAZ | ||
---|---|---|---|---|---|
KPC-2 | k cat (sec-1) | 50 ± 7 | 48 ± 3 | 3.8 ± 0.7 | |
K m (μM) | 226 ± 68 | 252 ± 30 | 36 ± 16 | ||
k cat/Km (μM-1.sec-1) | 0.23 ± 0.04 | 0.19 ± 0.03 | 0.11 ± 0.036 | 0.0008 ± 0.0002 | |
P104R | k cat (sec-1) | 314 ± 15 | 28 ± 1 | 3.0 ± 0.4 | |
(KPC-5) | K m (μM) | 1407 ± 123 | 247 ± 22 | 53 ± 10 | |
k cat/Km (μM-1.sec-1) | 0.22 ± 0.01 | 0.12 ± 0.01 | 0.06 ± 0.004 | 0.009 ± 0.001 | |
P104L | k cat (sec-1) | 12.8 ± 0.2 | 54 ± 4 | 2.5 ± 0.1 | |
(KPC-11) | K m (μM) | 136 ± 18 | 244 ± 31 | 35 ± 7 | |
k cat/Km (μM-1.sec-1) | 0.1 ± 0.01 | 0.22 ± 0.01 | 0.07 ± 0.01 | 0.002 ± 0.0002 | |
V240G | k cat (sec-1) | 146 ± 4 | 27 ± 1 | 2.3 ± 0.07 | |
(KPC-6) | K m (μM) | 318 ± 11 | 172 ± 23 | 30 ± 4 | |
k cat/Km (μM-1.sec-1) | 0.46 ± 0.01 | 0.16 ± 0.02 | 0.08 ± 0.008 | 0.004 ± 0.0005 | |
H274Y | k cat (sec-1) | 224 ± 16 | 34 ± 4 | 1.6 ± 0.1 | |
(KPC-3) | K m (μM) | 432 ± 48 | 108 ± 25 | 31 ± 8 | |
k cat/Km (μM-1.sec-1) | 0.52 ± 0.02 | 0.32 ± 0.04 | 0.05 ± 0.019 | 0.007 ± 0.0003 | |
P104R:V240G | k cat (sec-1) | 61 ± 5 | 21 ± 1 | 2.3 ± 0.04 | |
(KPC-4) | K m (μM) | 538 ± 84 | 157 ± 18 | 30 ± 3 | |
k cat/Km (μM-1.sec-1) | 0.12 ± 0.01 | 0.14 ± 0.01 | 0.08 ± 0.006 | 0.04 ± 0.002 | |
P104R:H274Y | k cat (sec-1) | 190 ± 3 | 31 ± 1 | 3.1 ± 0.07 | |
(KPC-10) | K m (μM) | 633 ± 42 | 211 ± 23 | 36 ± 1 | |
k cat/Km (μM-1.sec-1) | 0.3 ± 0.02 | 0.15 ± 0.01 | 0.08 ± 0.003 | 0.06 ± 0.01 | |
V240A:H274Y | k cat (sec-1) | 49 ± 4 | 27 ± 3 | 2.2 ± 0.2 | |
(KPC-9) | K m (μM) | 148 ± 24 | 113 ± 28 | 21 ± 2 | |
k cat/Km (μM-1.sec-1) | 0.33 ± 0.03 | 0.24 ± 0.04 | 0.1 ± 0.001 | 0.02 ± 0.004 | |
V240G:H274Y | k cat (sec-1) | 24 ± 0.3 | 24 ± 2 | 2.6 ± 0.2 | |
(KPC-8) | K m (μM) | 132 ± 23 | 110 ± 22 | 17 ± 2 | |
k cat/Km (μM-1.sec-1) | 0.18 ± 0.03 | 0.22 ± 0.02 | 0.16 ± 0.007 | 0.03 ± 0.003 | |
M49I:H274Y | k cat (sec-1) | 225 ± 26 | 32 ± 1 | 3.0 ± 0.1 | |
(KPC-7) | K m (μM) | 411 ± 108 | 120 ± 1 | 31 ± 3 | |
k cat/Km (μM-1.sec-1) | 0.56 ± 0.08 | 0.26 ± 0.01 | 0.1 ± 0.006 | 0.006 ± 0.0002 |