Table 3. Kinetic parameters of KPC variants.

		AMP	IMI	MERO	CAZ
KPC-2	k cat (sec-1)	50 ± 7	48 ± 3	3.8 ± 0.7
	K m (μM)	226 ± 68	252 ± 30	36 ± 16
	k cat/Km (μM-1.sec-1)	0.23 ± 0.04	0.19 ± 0.03	0.11 ± 0.036	0.0008 ± 0.0002
P104R	k cat (sec-1)	314 ± 15	28 ± 1	3.0 ± 0.4
(KPC-5)	K m (μM)	1407 ± 123	247 ± 22	53 ± 10
	k cat/Km (μM-1.sec-1)	0.22 ± 0.01	0.12 ± 0.01	0.06 ± 0.004	0.009 ± 0.001
P104L	k cat (sec-1)	12.8 ± 0.2	54 ± 4	2.5 ± 0.1
(KPC-11)	K m (μM)	136 ± 18	244 ± 31	35 ± 7
	k cat/Km (μM-1.sec-1)	0.1 ± 0.01	0.22 ± 0.01	0.07 ± 0.01	0.002 ± 0.0002
V240G	k cat (sec-1)	146 ± 4	27 ± 1	2.3 ± 0.07
(KPC-6)	K m (μM)	318 ± 11	172 ± 23	30 ± 4
	k cat/Km (μM-1.sec-1)	0.46 ± 0.01	0.16 ± 0.02	0.08 ± 0.008	0.004 ± 0.0005
H274Y	k cat (sec-1)	224 ± 16	34 ± 4	1.6 ± 0.1
(KPC-3)	K m (μM)	432 ± 48	108 ± 25	31 ± 8
	k cat/Km (μM-1.sec-1)	0.52 ± 0.02	0.32 ± 0.04	0.05 ± 0.019	0.007 ± 0.0003
P104R:V240G	k cat (sec-1)	61 ± 5	21 ± 1	2.3 ± 0.04
(KPC-4)	K m (μM)	538 ± 84	157 ± 18	30 ± 3
	k cat/Km (μM-1.sec-1)	0.12 ± 0.01	0.14 ± 0.01	0.08 ± 0.006	0.04 ± 0.002
P104R:H274Y	k cat (sec-1)	190 ± 3	31 ± 1	3.1 ± 0.07
(KPC-10)	K m (μM)	633 ± 42	211 ± 23	36 ± 1
	k cat/Km (μM-1.sec-1)	0.3 ± 0.02	0.15 ± 0.01	0.08 ± 0.003	0.06 ± 0.01
V240A:H274Y	k cat (sec-1)	49 ± 4	27 ± 3	2.2 ± 0.2
(KPC-9)	K m (μM)	148 ± 24	113 ± 28	21 ± 2
	k cat/Km (μM-1.sec-1)	0.33 ± 0.03	0.24 ± 0.04	0.1 ± 0.001	0.02 ± 0.004
V240G:H274Y	k cat (sec-1)	24 ± 0.3	24 ± 2	2.6 ± 0.2
(KPC-8)	K m (μM)	132 ± 23	110 ± 22	17 ± 2
	k cat/Km (μM-1.sec-1)	0.18 ± 0.03	0.22 ± 0.02	0.16 ± 0.007	0.03 ± 0.003
M49I:H274Y	k cat (sec-1)	225 ± 26	32 ± 1	3.0 ± 0.1
(KPC-7)	K m (μM)	411 ± 108	120 ± 1	31 ± 3
	k cat/Km (μM-1.sec-1)	0.56 ± 0.08	0.26 ± 0.01	0.1 ± 0.006	0.006 ± 0.0002