Fig 6. Trypsin activity in P. argentipes, P. orientalis, P. papatasi and S. schwetzi midguts.

Trypsin activity was measured at 12, 24, 36, 48, 72, 80, and 96 hours PBM in midgut homogenates (c = 0.005 gut/ml) of bloodfed females using fluorogenic substrate Boc-Leu-Gly-Arg-AMC (40 μM). Data from two independent experiments were pooled. 6A: Females fed on anesthetized BALB/c mice. 6B: Females fed through a chick-skin membrane on heat-inactivated rabbit blood.